FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23956761
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG41650.09.3512974
GTAGAAC28950.08.93682911
CACTCGT58350.08.905663535
GGACTTA37950.08.3646574
ACTCGTG63350.08.23846936
GCGGAAA69200.08.2325461
CTCTATA63700.08.19258745
TCACTCG65000.08.02962334
TACTCGA48200.07.84695675
AGTACTC52050.07.74306733
GGAAAAT96350.07.66552451
GACTTAT40900.07.5411275
GAATCGA38900.07.5237534
AAATCCA99950.07.3880844
AATCGAA48100.07.34841255
AAAATCC75650.07.29185343
AAAAATC79100.07.2875782
CGACGAT90000.07.2806521
GGAGAAT70250.07.2440571
GGTAGAA38500.07.129299610
Produced by FastQC (version 0.11.5)