FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17449136
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC24350.011.91873311
GTACTCG39500.011.399284
TACTCGA41800.010.6642655
CGTGACT16550.010.47639752
AGTACTC46000.010.1810463
CTCGAAT15200.09.925571
AATCGAA31050.08.700855
GGTAGAA32550.08.5706510
ACACGCG17600.08.5650296
GCGTGAC21350.08.5430191
GAATCGA27800.08.5033294
GAATTGA39250.08.3744774
TAGAACA33600.08.36922412
TCAGGTA37400.08.3017657
TATTCGG16600.08.13125512
GGTAGGC15600.08.076567
CTCTATA38750.08.06896245
GGAAAAT77050.07.9783711
GCATAGG25450.07.955120641
CACTCGT38750.07.953180335
Produced by FastQC (version 0.11.5)