FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30089003
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC38750.08.99898211
GGAAAAT148300.08.684941
AAAATCC111900.07.96785933
AATTCAC58150.07.85927445
AAAAATC112100.07.85384652
GGGGAAT67200.07.84076741
CCCGAAT24250.07.79972931
AAATCCA121150.07.377414
GGAAGAT215600.07.24807551
GTACTCG44200.06.9780674
CGCGATA14900.06.947614729
GGCGAAT30500.06.9396891
CGAAAAT45500.06.77986671
TAATCGA32250.06.77140574
AAATCCT116300.06.75588944
GAAAATC126950.06.7400352
AATCGAG58000.06.7151275
AATCGAA65250.06.69356255
AGATCCA142700.06.6734934
TCAGGTA61650.06.64211567
Produced by FastQC (version 0.11.5)