FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27621332
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCAC63150.010.0175725
AAAATCC97250.08.6354813
AAAAATC99350.08.63487152
GAATTCA111200.07.89570474
AACCTAC43100.07.777082412
GGGGAAT56950.07.74926231
GGAAAAT131300.07.69980341
AATCCAA131200.07.53286555
CTTGAAT88950.07.51810741
TTGAATT95450.07.2892252
GGAAGAT192800.07.2640921
CCCGAAT23250.07.2633391
AAATCCA107650.07.25682264
GGGAAAT80850.07.1294811
AATCGTA18950.07.1280635
AAATCCT101900.07.025614
AATCCTA51150.06.9981145
TACAAAC50300.06.97637316
GAAAAAT137800.06.9444481
ATTCACA72250.06.9133936
Produced by FastQC (version 0.11.5)