FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34616156
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT32050.014.2620812
GTACTCG90800.013.190474
TACTCGA93400.012.65455
AGTACTC100450.012.2384073
GCGTGAC44900.010.9326711
AAAATCC115350.09.3107153
GGAAAAT155750.09.2671481
CTCTATA77850.09.21737945
GTAGAAC39150.09.19440911
GTGACTC52800.09.1256133
AAATCCT124950.08.8106324
CACTCGT79550.08.73819835
GCGGAAA88150.08.7106031
ACTCGAG148850.08.600726
ACTCGTG81600.08.57394536
GAATCGA48900.08.5632494
AATCCTT107900.08.3458615
AGTGTGA77750.08.15865314
GTGTGAG77600.08.02956715
AAGTACT159850.07.93068362
Produced by FastQC (version 0.11.5)