FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29226336
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29600.012.3231462
GTACTCG61900.011.3836644
TACTCGA66350.010.4504915
AGTACTC70800.010.3032883
GTAGAAC33600.010.110579511
GCGTGAC40000.09.7383611
GGAAAAT129450.09.6884191
AATTGAC48800.09.0419715
AATCGAA51650.08.8045655
GAATCGA38750.08.7727064
AAAATCC94900.08.6120093
CTCTATA66350.08.61125645
CACTCGT68800.08.33772835
ACTCGTG70650.08.11956836
GTGACTC46400.08.0547893
CCAGTGT65100.08.05176412
GAATTGA73950.08.0370034
TCACTCG70050.07.83615434
TCACACG26450.07.8242615
CTCGAAT23500.07.7609871
Produced by FastQC (version 0.11.5)