FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19071788
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG14650.027.78395815
CGTCTGA15200.026.63267116
CACACGT18600.022.35670312
CACGTCT19100.021.89424114
ACACGTC20350.020.32422313
TCGGAAG27500.018.5399683
ATCGGAA27500.018.4586052
CAGTCAC17850.018.41948527
ACCGATC10000.017.45407734
AGCACAC25600.016.59286710
CCGAGAC10900.016.35748329
GATCGGA31850.016.0161821
GAGACCG11050.015.97566831
AGCGGTT12300.015.44975611
GCACACG27750.015.22672911
CGGAAGA34350.015.1031694
CCAGTCA23450.014.29500926
CGGGCTA1758.7257285E-714.063331
AACTCCA31800.013.31900722
GGAATGC14600.013.05072123
Produced by FastQC (version 0.11.5)