FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25142216
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44050.021.93366243
CTCGTAT44700.021.76565242
AGTCACG53400.021.31491528
ACGTCTG56300.020.7763515
CGTCTGA59600.019.47501416
CGTATGC52250.017.97419744
ATCGGAA71000.016.984962
GTCACGA69600.016.709229
TCGGAAG72750.016.6073153
CACGTCT77000.015.83371814
CACACGT79450.015.48701712
CAGTCAC86700.014.03629527
ACACGTC91050.013.58804813
TCACGAT87800.013.32242130
TCTCGTA79100.012.92595841
GATCGGA95550.012.6696611
ACGATCA97500.012.25130632
TCAGATC96500.012.19536136
GTATGCC82350.011.86939245
GTCTGAA101600.011.69002317
Produced by FastQC (version 0.11.5)