FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25483476
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59400.015.56420315
CGTCTGA61150.015.08199916
GTCACAC66400.013.24586129
TCGTATG26750.013.05038843
CTCGTAT30700.012.61823942
TATAGGC53150.012.56969427
TCGGAAG78000.012.0282043
CACGTCT82300.011.5067814
CAGTCAC80100.011.37349827
ATCGGAA82600.011.3039192
AGTCACA80850.011.184525528
TTATAGG60250.010.97644726
ACTATTA62600.010.78014119
TACTATT66150.010.3716418
CACACGT90100.010.33587112
CACACTT87150.010.27288231
CTATTAT66800.010.13602220
CTGGATT65650.09.9740631
CGCGTAT11100.09.73043251
ACACGTC99150.09.71007413
Produced by FastQC (version 0.11.5)