FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20298362
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41150.018.03939415
CGTCTGA43950.016.83894316
CTCGTAT30700.016.65219942
TCGTATG30300.016.50056343
TCGGAAG54200.014.7368363
ATCGGAA54700.014.0674042
CACGTCT55350.013.61460414
CGTATGC37800.012.98832444
CACACGT64700.012.23816312
CAGTCAC62850.012.13311327
ACACGTC65500.012.08868913
AGTCACC65150.011.94646728
TATAGGC37150.011.26242627
GATCGGA70950.011.22707751
CGGAAGA76400.010.7197154
CGCTATG15000.010.64743429
ACTATTA45000.010.09766519
TTATAGG43850.09.79809426
GTATGCC52850.09.758430545
CCCGACT21300.09.6134141
Produced by FastQC (version 0.11.5)