FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23956761
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG52550.024.05690228
ACGTCTG58300.022.8031615
CTCGTAT47500.022.28416642
TCGTATG46650.021.7732143
CGTCTGA62450.021.10771816
GTCACGC64550.019.68920929
CGCCAAT67350.018.83855233
ACGCCAA68800.018.60403832
TCGGAAG73050.018.4797843
ATCGGAA72550.018.4210682
CGTATGC54250.018.26629844
CACGTCT74550.018.04386714
TATCTCG65300.017.79053139
CAGTCAC79250.016.68991727
CACGCCA78250.016.58702531
CACACGT82800.016.40902312
TCACGCC81900.016.36961630
ACACGTC84350.016.18749613
GCCAATA84250.015.16751334
GATCGGA95250.014.2692541
Produced by FastQC (version 0.11.5)