FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17449136
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG28800.022.2599615
TCGTATG27200.021.94279743
CGTCTGA31550.020.24841516
CTCGTAT29350.020.02787842
CGTATGC32650.018.41802644
TCGGAAG41250.016.1995013
CACGTCT42600.015.73543414
ATCGGAA43050.015.365382
GTCACAC41900.015.19303329
CAGTCAC47550.013.34045427
CACACGT50800.013.19546112
AGTGATC47700.012.87821736
ACACGTC55200.012.55115513
CACAGTG50600.012.53716333
AGTCACA51250.012.07010128
GTATGCC53950.011.81435445
TCTCGTA52750.011.74076941
ACACAGT54850.011.401319532
GATCGGA58600.011.1742731
CGGAAGA63850.010.7122774
Produced by FastQC (version 0.11.5)