FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30089003
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG77350.022.30508815
CGTCTGA80400.021.2910716
CTCGTAT54950.020.32922742
TCGTATG55500.019.2758743
TCGGAAG93850.018.3637283
ATCGGAA93500.018.2640252
CACACGT95050.018.08048812
AGTCACT94200.017.62277828
CGTATGC58450.017.49382244
CACGTCT103700.017.00615714
ACTGACC101300.016.67684232
ACACGTC107200.016.38796813
GATCGGA111950.015.35646251
AATCTCG99350.014.6625339
CAGTCAC117750.014.461184527
GTCTGAA122700.014.26286617
GTCACTG119850.014.1702629
CACTGAC122900.013.74552331
CGGAAGA133600.013.2031014
GTATGCC82850.013.02138245
Produced by FastQC (version 0.11.5)