FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27621332
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG67300.021.5917415
ACTTAGG67700.021.199232
CGTCTGA71450.020.55801216
TCGTATG48600.020.53000643
CTCGTAT52450.020.13929442
TCGGAAG81000.018.6378463
CGTATGC54350.018.31657844
ATCGGAA82750.018.1349032
TTAGGCA79500.017.94180934
CACTTAG79750.017.74171631
TCACTTA79300.017.64360630
CACGTCT89350.016.89266614
AGTCACT86950.016.58282928
GTCACTT87850.016.28491629
CTTAGGC89300.016.27385933
CACACGT93750.016.09983412
ACACGTC97200.015.50524713
GATCGGA99600.015.2044991
TAGGCAT98350.014.66526435
CAGTCAC108650.013.51928127
Produced by FastQC (version 0.11.5)