FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34616156
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC523940.15135707153619252TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG84700.030.2073543
CTCGTAT91550.029.22588242
ACGTCTG138400.026.03742615
CGTATGC96750.025.9329544
CGTCTGA139850.025.55836316
TATCTCG122150.025.49118639
GATGTAT134400.025.347635
CACCGAT145350.024.01884331
ACCGATG146700.023.8596632
CACGTCT162800.022.28699714
CAGTCAC161800.021.75742327
TGTATCT158800.021.55515337
TCGGAAG170000.021.5457273
ATCGGAA169800.021.4651362
GTATCTC160000.021.31331638
CACACGT175000.020.83610512
AGTCACC170750.020.761928
TCTCGTA143050.019.17595741
ACACGTC192150.019.0349513
CCAGTCA192800.018.45742226
Produced by FastQC (version 0.11.5)