FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n01_S2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n01_S2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29226336
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC637290.21805333381509062TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG124600.033.2362115
CGTCTGA127200.032.6629616
CTCGTAT100450.032.5990342
TCGTATG103650.032.4187643
CGTATGC109950.030.02863744
TCGGAAG141800.029.6711243
CACGTCT141800.029.47441914
ATCGGAA145450.028.84922
CACACGT151700.027.62504412
CAGTCAC147950.027.54983327
GTCACAT147500.027.54238129
ACACGTC160950.026.30294413
AGTCACA154950.026.14555528
TCACGAT154750.025.72207335
GATCGGA167550.025.2080361
ATCACGA165100.024.3378934
TCTCGTA138450.024.15505841
CATCACG172550.023.67609433
CCAGTCA174200.023.50168426
CGGAAGA181550.023.4721224
Produced by FastQC (version 0.11.5)