FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16565913
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT9650.022.3909032
GAGTGTA6050.020.83177228
AGTGTAG6250.020.16582529
TAGATCT6950.019.10150533
TCTCGGT6650.018.6079937
GTAGATC7400.018.2468632
GTGTAGA7750.018.00471130
TGTAGAT9450.014.28915131
ATCGGAA17350.013.7509932
GATCTCG10700.013.03437635
TCGGAAG18700.012.637763
CTCGGTG10900.012.59104438
GATCGGA19200.012.543521
TCCCTAC6150.012.4373959
TGTAGGG10600.012.30918818
AGCGGTT8100.012.22062110
GTGTAGG11100.012.15972317
AGATCTC10750.012.13640534
TAGGGAA11300.011.74685220
TCGTGTA10950.011.70955715
Produced by FastQC (version 0.11.5)