FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21667859
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC28450.012.33540811
CGTGACT20550.012.2674462
GTACTCG65550.012.0857824
AGTACTC68800.011.8102673
TACTCGA67300.011.5033715
GCGTGAC27400.09.8579751
CTCGAAT18250.09.743641
CACTCGT45400.09.36416435
GAATCGA31850.09.3275874
CTCTATA46200.09.25053145
ACTCGAG92050.09.1901046
AATCGAA38450.09.1308255
ACTCGTG47500.08.95147236
GCGGAAA53650.08.8945321
GGTAGAA41300.08.87891310
GTGACTC33200.08.745663
TCACTCG49000.08.58427434
GGAAAAT93500.08.4980661
TAGAACA40300.08.4849612
TCAGGTA44850.08.4272947
Produced by FastQC (version 0.11.5)