FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23854871
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG50600.012.187164
CGTGACT20550.012.0483872
TACTCGA52750.011.4337895
AGTACTC58900.010.8529183
GTAGAAC30150.010.52048111
TATTCGC39100.09.7815557
TAGGCGG36950.09.74532131
GCATAGG38800.09.50856641
AATCGAA42150.09.397085
GCGTGAC28800.09.3786721
GTAGGCG38250.09.17902430
GGAAAAT104100.09.1462321
TCGTAGG39550.08.99155828
GCGGAAA58500.08.9265721
ATCGTAG40750.08.89221327
CACTCGT51350.08.8486235
AAAATCC80550.08.6624283
CTCTATA53600.08.64482445
ATTCGCA49250.08.5878168
CATAGGA43050.08.56960742
Produced by FastQC (version 0.11.5)