FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22924870
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36500.013.37446811
GTACTCG40700.010.506354
GGTAGAA47700.010.18723610
TCAGGTA50400.010.1329367
TAGAACA51500.09.39164812
TACTCGA45700.09.1102875
AGTACTC55850.08.8659513
CGACGAT87300.08.5080921
CAGGTAG58800.08.4174878
GGAAAAT91350.08.3033581
AATCGAA40750.08.2845
AGATCAG91950.08.1749934
AAAATCC69250.08.1254233
CTCGAAT21900.08.1192411
CGAGAAT34050.07.8661511
GAATCGA38750.07.8406974
CGAAAAC46600.07.77628951
CGACAAT32000.07.7370461
GGAAGAT136050.07.6266671
ACATGCG26650.07.59803636
Produced by FastQC (version 0.11.5)