FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22652091
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG37850.010.10856154
GTAGAAC29850.010.02342111
CACTCGT52200.09.65233735
CTCTATA53850.09.48187445
ACTCGTG56750.09.11753436
GCGGAAA60800.08.9589191
TACTCGA42450.08.906745
TCACTCG57000.08.87883434
CTCGAAT18600.08.59191
GTAGGCG26300.08.47190430
CGGCTAT25000.08.2830761
CGGAAAC64950.08.2478672
GCATAGG28400.08.23781841
AGTACTC48700.08.2267663
GGAAAAT87300.08.0184411
GGTAGAA39450.07.983645410
TATTCGC30900.07.93607047
CGTATAG15600.07.931344512
TCGTAGG27850.07.91995328
TAGGCGG26750.07.90853531
Produced by FastQC (version 0.11.5)