FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26086954
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC40300.011.0526411
CGTGACT21600.010.5246692
GTACTCG61800.010.23366454
AGTACTC71750.09.2855073
TACTCGA69800.08.8991365
GAATCGA40250.08.8349594
GCATAGG38750.08.76597741
GCGTGAC28750.08.69020751
TAGGCGG39000.08.42524231
TAGAACA53450.08.41762912
GGTAGAA53200.08.33061610
TCAGGTA57950.08.3082027
CTCGAAT20750.08.2440471
TAGTGTA21350.08.22302625
CTCTATA58850.08.17938645
GGTAGGC21750.08.06837
AATCGAA47300.07.9935985
TATTCGC43950.07.98568967
GGAAAAT105700.07.90030571
ATAGGAG44500.07.835004343
Produced by FastQC (version 0.11.5)