FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22612320
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22850.013.593462
GTACTCG52200.013.2796194
TACTCGA55050.012.2236265
AGTACTC61200.011.7319623
GTAGAAC28450.011.38655711
GCGTGAC31550.010.62986851
GCATAGG29050.010.06692741
TAGGCGG28600.09.83630831
GTAGGCG30750.09.36840130
GTGACTC35450.09.2062593
CGGCTAT24700.09.1126371
GCGGAAA60100.08.9883041
TTACGAC19750.08.88884629
GGAAAAT93750.08.8352241
CACTCGT51550.08.81416335
ACTCGAG83500.08.6491626
AGCCTAG28400.08.55932925
CTCTATA53150.08.50637345
TCGTAGG33950.08.48578428
ATCGTAG33700.08.41521627
Produced by FastQC (version 0.11.5)