FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26757211
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23150.012.6396072
GTACTCG57100.011.1150654
AGTACTC63900.010.5671223
TACTCGA63300.09.9904385
GTAGAAC31700.09.50928711
GCGGAAA71750.09.254461
GCGTGAC33200.09.2204441
GAATCGA39650.08.9115944
TATTCGC39200.08.7234847
CTCTATA67650.08.71138445
CACTCGT68400.08.6160935
AATCGAA46500.08.5664765
GCATAGG38350.08.56419741
GTGACTC38650.08.3276593
GGAAAAT103550.08.216611
TAGGCGG38850.08.16819831
ACTCGTG71750.08.120893536
TCACTCG72400.08.07768334
GAATTGA65450.08.0120794
GTAGGCG39350.07.95015530
Produced by FastQC (version 0.11.5)