FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29098913
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG57450.011.4784424
CGTGACT28100.011.1338082
TACTCGA61900.010.3255435
AGTACTC69500.010.1042023
GCGGAAA77900.09.8238731
AGCCTAG36200.09.637135525
GCGTGAC36750.09.4932641
CTCTATA71250.09.43927245
CACTCGT73200.09.28019335
ACTCGTG75600.09.13568736
GGAAAAT111100.08.6102561
TCACTCG79300.08.56619934
CGGAAAC87150.08.49696352
AATCGAA52600.08.3004415
TAGAAAC44000.08.23133417
AAGCCTA44350.08.11986724
GCTAGTT89700.07.97527812
TCACACG28950.07.925477515
GAATTGA68800.07.8183454
GCTCTAT90350.07.817243644
Produced by FastQC (version 0.11.5)