FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n02_C2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n02_C2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25035355
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG59950.012.539024
TACTCGA62150.012.05839355
AGTACTC69600.011.1891823
GTAGAAC30750.010.09581711
CGTGACT22400.09.4453542
GGAAAAT107250.09.2131531
TTACGAC24800.09.07529429
AAAATCC78250.08.45657353
GAATCGA38000.08.2918364
TAGGCGG32250.08.23455931
GCATAGG34250.08.21007941
ACTCGAG104800.08.050646
TATTCGC37550.08.0285497
GTAGGCG33600.07.83689830
AATTGAC39400.07.82547475
GAAAATC91850.07.79267172
CGCGAAT27800.07.768271441
AATCGAA43350.07.68349745
CGGCTAT29600.07.6801681
TCACACG26450.07.65395915
Produced by FastQC (version 0.11.5)