FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16565913
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG13350.033.15564315
CGTCTGA13900.031.84873216
CACACGT15300.029.20803512
CACGTCT15400.029.01837214
ACACGTC16300.027.55321113
CAGTCAC12450.025.69819527
AGCACAC18400.024.4085510
GCACACG20250.022.50965911
TCGGAAG23250.022.4925793
ATCGGAA23750.022.1139492
CCAGTCA15300.021.94037626
CGGAAGA25800.020.5293394
GAGACCG6150.020.21849431
GATCGGA26150.020.2105331
AACTCCA21550.019.43458222
CTCCAGT21650.017.84470224
CCGAGAC7000.017.73182329
CTGAACT27350.015.94112719
AGCGGTT8700.015.92341511
ACCGATC7650.015.43457334
Produced by FastQC (version 0.11.5)