FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21667859
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT39300.022.86694142
AGTCACG43050.022.8339228
TCGTATG39550.022.6087143
ACGTCTG45800.022.15048415
CGTCTGA48800.020.7426816
GTCACGA56200.017.81130829
CGTATGC50150.017.60543844
CACGTCT60800.017.20368814
TCGGAAG59950.017.1888453
ATCGGAA61350.016.7232882
CACACGT64050.016.19030212
CAGTCAC64950.015.51568627
TCACGAT70500.014.134706530
ACACGTC77650.013.81812413
TCTCGTA67000.013.61403641
CGCGTAT10050.012.7624571
ACGATCA80850.012.71482432
AGCACAC86200.012.65632410
GTATGCC73150.012.56254345
GATCGGA85700.012.3670361
Produced by FastQC (version 0.11.5)