FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23854871
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG63200.020.85736515
CGTCTGA66800.019.93536216
TCGTATG35150.019.41450543
CTCGTAT37100.018.94030442
GTCACAC73800.017.55678429
TCGGAAG81000.016.6664263
ATCGGAA80650.016.6272342
CACGTCT84000.015.88013814
CACACGT84400.015.80487812
AGTCACA83800.015.56907728
CGTATGC41250.015.50612944
CAGTCAC84850.015.48245727
CACACTT90200.014.43948831
AATCTCG66550.013.70107539
ACACGTC99200.013.628313
TTGAATC96400.013.56057636
TCACACT98150.013.40739630
GATCGGA106000.012.843171
ACTTGAA105750.012.76521234
CGGAAGA110550.012.6796024
Produced by FastQC (version 0.11.5)