FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22924870
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT38600.017.21394742
ACGTCTG50200.016.71374115
TCGTATG38050.016.63424743
CGTCTGA52850.016.08849116
CGTATGC45100.013.93410644
TCGGAAG66650.013.1313933
CACGTCT66450.012.76188714
ATCGGAA69100.012.3727692
TATAGGC39050.012.26950627
CACACGT70200.012.20833312
CAGTCAC73050.011.63965527
AGTCACC77350.011.25431728
ACACGTC78350.011.08196613
TTATAGG47150.010.35253126
GTATGCC64450.010.30980845
GATCGGA84850.010.209921
ACTATTA47700.010.044641519
ATACTAT50550.09.83425717
CGGAAGA91200.09.6952514
CTGGATT63150.09.6919021
Produced by FastQC (version 0.11.5)