FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22652091
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG43550.023.19174428
ACGTCTG47450.021.90185715
CTCGTAT39900.021.50671442
CGTCTGA49250.020.96436116
TCGTATG41550.020.38180443
GTCACGC53350.019.01591929
TATCTCG52800.017.9089639
TCGGAAG60700.017.2730583
ATCGGAA61600.016.9841672
CGTATGC50400.016.9816144
CACGTCT64700.016.37539714
ACGCCAA60600.016.29558632
CGCCAAT61750.016.065633
CACACGT66550.016.05538612
CAGTCAC70950.014.9011827
CACGCCA69000.014.76815531
TCACGCC73000.014.76008330
ACACGTC75850.014.17579813
GCCAATA73000.013.74612234
GATCGGA80200.013.2992991
Produced by FastQC (version 0.11.5)