FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26086954
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC744830.2855181942667588TruSeq Adapter, Index 5 (100% over 51bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA298320.1143560110544144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG107800.033.30248343
CTCGTAT107900.033.12531742
ACGTCTG124250.030.68690715
CGTCTGA127250.029.91041216
CGTATGC121100.029.57059344
GTCACAC136300.027.14875229
TCGGAAG146050.026.4819433
CACACGT145150.026.43880512
CACGTCT145350.026.40242414
ATCGGAA148500.025.984532
CAGTCAC142900.025.95782327
AGTCACA143150.025.70820628
GTATGCC140700.025.62738445
AGTGATC143650.025.01361836
CACAGTG146000.024.96061533
TCTCGTA147050.024.48821841
ACACGTC160150.024.1310413
ACACAGT153400.023.87328332
CAGTGAT159100.022.96574835
CACACAG165400.022.48116731
Produced by FastQC (version 0.11.5)