FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22612320
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41850.022.03801715
CGTCTGA43250.021.3766616
TCGTATG35150.019.99054343
CTCGTAT35550.019.51220942
AGTCACT53300.017.13495428
ATCGGAA56100.016.9647372
TCGGAAG57900.016.5539113
CACGTCT59400.015.79187714
ACTGACC59550.015.18564432
CGTATGC44200.015.18410944
CACACGT65050.014.62773812
CAGTCAC67350.013.72740227
AATCTCG60900.013.27113639
ACACGTC74000.013.19295713
GTCACTG73650.012.85859429
CACTGAC76300.012.20570431
CGGAAGA83500.011.9906624
GATCGGA81650.011.7950931
TGACCAA79300.011.66101534
CTGACCA84200.011.32797633
Produced by FastQC (version 0.11.5)