FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26757211
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC282540.10559396493154687TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG55350.025.02536843
CTCGTAT57150.024.51285442
ACGTCTG73600.023.31975715
CGTCTGA74700.023.09681116
ACTTAGG74700.022.5248332
CGTATGC63500.021.24594544
TCACTTA80050.020.65395730
TTAGGCA80650.020.53191434
AGTCACT83450.019.70459428
GTCACTT85100.019.58687429
TCGGAAG90850.019.3417933
ATCGGAA91450.019.0427072
CACGTCT92000.018.9247614
CACACGT97600.017.76977212
CTTAGGC98300.017.20876533
CAGTCAC98700.016.91076327
ACACGTC110300.016.1316313
GTATGCC90650.015.47901145
GATCGGA114950.015.230221
CGGAAGA119100.014.9806984
Produced by FastQC (version 0.11.5)