FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29098913
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1729950.5945067432587602TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG220000.036.4223443
CTCGTAT227100.036.27487642
CGTATGC225550.034.747344
TATCTCG274450.033.28703339
GTATGCC243250.032.37631245
GATGTAT303150.032.15946235
TGTATCT315700.030.70485937
GTATCTC319050.030.26378438
CACCGAT328950.030.13672331
ACCGATG330650.029.93420632
TCTCGTA277550.029.90687841
CGTCTGA348150.029.05628416
ACGTCTG353100.028.7126615
CAGTCAC348350.028.6456427
AGTCACC355100.028.17080928
ATGTATC345350.028.14562236
ATCTCGT318950.027.37113640
CGATGTA359200.027.30958434
CACGTCT373700.027.20814314
CACACGT384450.026.52350812
Produced by FastQC (version 0.11.5)