FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l07_n01_C2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l07_n01_C2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25035355
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC520410.2078700302032865TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT80800.032.3057842
TCGTATG83300.032.22883243
ACGTCTG101500.030.87193115
CGTCTGA103050.030.23294816
CGTATGC91850.028.90999444
TCGGAAG117450.027.0876523
ATCGGAA118050.026.8737952
CACGTCT119150.026.44982314
CACACGT121150.026.16177212
GTCACAT119750.025.56591429
CAGTCAC120450.025.52938827
TCACGAT119500.025.45567735
AGTCACA123800.024.7658928
ACACGTC134700.023.64690813
ATCACGA130150.023.45864334
TCTCGTA115050.023.21570441
GATCGGA142450.022.415291
CATCACG139200.022.285333
CCAGTCA138500.022.20227426
GTATGCC123750.021.87623645
Produced by FastQC (version 0.11.5)