FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17114657
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT9800.022.2869762
GAGTGTA6550.021.9900228
GTGTAGA6800.021.84029430
AGTGTAG6700.021.8319529
TCTCGGT7200.021.24417937
GTAGATC7100.020.91575232
TAGATCT7450.019.9305133
TGTAGGG9900.015.67944518
GTGTAGG10050.015.6692717
GATCTCG10350.015.64834135
TCGTGTA9950.015.59946915
TGTAGAT9400.015.55884131
TCCCTAC6500.015.228029
GTAGGGA9900.015.22541419
CTCGGTG10700.014.92690638
TAGGGAA10100.014.47893920
TTCCCTA8350.013.74035558
TACACGA6400.013.00443713
TCGGTGG11800.012.96330739
AGATCTC12600.012.6759534
Produced by FastQC (version 0.11.5)