FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19220969
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC27500.010.71587511
CGCGATA8900.09.6085529
CGTGACT18900.09.4124892
CGATAGT9750.09.23099231
AAAATCC59000.09.0070423
CACTCGT32100.08.89937435
AAAAATC55200.08.8115812
TAGAACA34000.08.79888912
GGAAAAT83050.08.7309421
GTACTCG38650.08.4471934
TCAGGTA39950.08.4467037
CTCTATA35300.08.28437845
GCGTGAC24550.08.2553571
TCACTCG34750.08.15630534
TACTCGA38100.08.1549635
TCCGTTA19600.08.14949919
ACTCGTG35400.08.13329236
CGTTAAC18600.08.10433921
AATTCAC33000.07.9825165
CCGTTAA21650.07.897855320
Produced by FastQC (version 0.11.5)