FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24686305
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25800.013.7024082
GTACTCG57100.013.2883514
AGTACTC62700.012.1738763
TACTCGA61650.011.9783735
GTAGAAC31850.011.86569711
GCGTGAC34500.011.0956321
GCGGAAA59900.010.3753911
CTCTATA55800.010.15912345
CACTCGT54450.010.03846835
GTGACTC40650.09.8595293
ACTCGTG57100.09.69076536
TCACTCG58150.09.40013634
TAGAACA41450.09.38806812
TCAGGTA47700.09.1966557
GGAAAAT100900.08.9490191
GGTAGAA43100.08.92508210
GAATCGA34800.08.8637734
AAAATCC74950.08.8022293
CGCGATA7450.08.76010729
AATTGAC41250.08.7327925
Produced by FastQC (version 0.11.5)