FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19475394
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG263740.1354221639880559Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG53400.024.02161229
GTAGATC55600.023.91649432
CGTGTAG55450.023.85308816
GAGTGTA54050.023.77595728
TCTCGGT56300.023.41178937
GTGTAGA56750.022.83929630
TGTAGGG59700.022.42117318
ATCTCGG62050.021.82261336
TCGTGTA62300.021.30293715
TAGATCT62600.021.2390733
GTGTAGG63750.021.13797817
GTAGGGA62800.020.88543519
TAGGGAA67200.019.58615320
TGTAGAT73000.018.12361731
AGAGTGT74250.017.7319727
ATCGGAA76500.017.601493
GTCGTGT79600.017.04039814
GATCTCG93050.016.70382735
AGCGTCG84450.016.7019811
GAGCGTC84500.016.66547410
Produced by FastQC (version 0.11.5)