FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26551795
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24450.012.7094892
GTAGAAC35700.011.40519311
GTACTCG45850.011.3438044
TACTCGA52150.010.1024035
CACTCGT84900.010.06782135
GCGTGAC34250.010.0592221
GCGGAAA92400.010.040581
CTCTATA86900.09.81090945
AGTACTC56500.09.7241213
ACTCGTG89300.09.647305536
TCACTCG91050.09.46223934
CTCGAAT22550.09.2868821
GAATCGA39400.09.0291514
GGTAGAA47250.08.76008410
CGGAAAC105050.08.6599052
TCGTGAG105350.08.58364138
AATCGAA50450.08.5238415
GCTAGTT105950.08.42836612
GGAAAAT100400.08.3209671
CTCGTGA108200.08.19067237
Produced by FastQC (version 0.11.5)