FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22673758
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23500.014.1811322
GTACTCG51750.012.4431014
GCGTGAC29700.011.5999441
TACTCGA55850.011.4080215
AGTACTC57700.011.3556293
GTAGAAC27250.010.64916411
CACTCGT60600.010.61609235
CTCTATA61000.010.21506945
GTGACTC35400.010.0495793
ACTCGTG64500.09.90439536
GCGGAAA68450.09.6386251
TCACTCG67100.09.62159134
CGCGATA8300.09.21871829
GGAAAAT84950.08.6677271
ACTCGAG79300.08.5966226
GCTAGTT78350.08.49789712
CGGAAAC77000.08.3928262
TCGTGAG81200.08.28343338
GCTCTAT78450.08.25830544
AAAATCC63350.08.1747653
Produced by FastQC (version 0.11.5)