FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26628213
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCAC65550.09.2734635
GGAAAAT128400.08.5582971
CGCGATA11950.08.47456629
AAAATCC91750.08.0740493
AAAAATC93450.08.0481122
AACCTAC46100.07.95319612
CTTGAAT85850.07.92135051
TTGAATT100100.07.8508782
AAATCCA106900.07.49798254
GAATTCA108300.07.33868744
AATTGAC48200.07.193245
ATTCACA80950.07.05961856
GGGGAAT61150.06.9598191
GAATTGA77400.06.9523094
AATCGTA15600.06.92735675
CGAAAAT38200.06.8969181
TACAAAC53950.06.79622116
GCGAAAT46600.06.76510761
AATCCAA131650.06.70372345
GAAAATC108350.06.62961962
Produced by FastQC (version 0.11.5)