FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22437467
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG43100.011.9630424
GTAGAAC32200.011.03817111
TACTCGA50050.010.3911175
AGTACTC54400.010.2237133
CGTGACT18850.09.0787972
AAAAATC63750.08.7245462
CGCGATA9650.08.62868629
AAAATCC76000.08.5327593
GGAAAAT101500.08.4969631
TCAGGTA46950.08.3374417
AATTCAC38150.08.3210655
TAGAACA43950.08.29104812
AATCGAA41100.08.1620415
GGTAGAA44150.07.94858510
GGGGAAT43100.07.6279271
CACTCGT37250.07.608541535
GAATTGA52350.07.56971364
CAGGTAG49400.07.51406868
GCGTGAC29400.07.50601961
GATCTAG40700.07.4125015
Produced by FastQC (version 0.11.5)