FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26388697
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG52650.011.8027784
GTAGAAC36000.010.56042211
TACTCGA60300.010.3049415
AGTACTC65750.09.7942093
CGTGACT20450.09.0289432
AAAATCC79350.08.5695713
ATCTAAC17100.08.2891166
GCGGAAA57550.08.2558021
GGAAAAT121350.08.2388261
CACTCGT48350.08.18804735
TCACTCG48250.08.15870734
ACTCGTG49700.08.101397536
AATCGAA50050.08.0969635
CTCTATA49850.08.07744945
TAGAACA48650.07.99872612
TCAGGTA53000.07.97992477
CATAAGC60400.07.85891067
CACGTAA34700.07.71409316
GGTAGAA51700.07.70157910
GAATTGA64100.07.692364
Produced by FastQC (version 0.11.5)