FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n02_B2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n02_B2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23149509
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG51500.011.3234954
AGTACTC59250.010.4890443
TACTCGA58650.010.1345635
CGTGACT22100.09.8836672
GTAGAAC26600.09.21807711
CACTCGT51600.08.80563135
GCGGAAA60200.08.7905041
AATCGAA37150.08.7271585
ACTCGTG52550.08.68922836
TCACTCG52650.08.4167734
CTCTATA53300.08.35660145
GAATCGA31750.08.2971724
GGAAAAT99050.08.2299371
ACACGCG21750.08.0685856
AAAATCC73050.07.9527013
GAATTGA54100.07.94921164
GCGTGAC31600.07.76751041
ATCTAAC12800.07.7340116
GTGACTC33750.07.60582353
AAATCCA83800.07.52318864
Produced by FastQC (version 0.11.5)