FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17114657
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG10200.030.04609315
CGTCTGA11150.027.48611316
CACGTCT11900.025.75251614
CACACGT13250.023.97264512
ACACGTC13800.023.82782213
ACCGATC6800.020.33965134
CAGTCAC10750.020.1285127
AGCACAC16100.019.727610
TCGGAAG20800.019.6826953
ATCGGAA20750.019.6224232
CCGAGAC7150.018.92365629
CCAGTCA12200.018.65994626
GAGACCG7150.018.64885331
GCACACG17900.017.99504711
CTCCAGT16000.017.94461824
GATCGGA22950.017.8025841
CGGAAGA24000.017.337984
AACTCCA19750.016.00603922
AGACCGA9500.014.99463832
AGCGGTT10450.014.1276911
Produced by FastQC (version 0.11.5)