FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19220969
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC205450.10688847164781337TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG43500.025.75189228
CTCGTAT39300.025.44451542
TCGTATG41200.024.70842243
ACGTCTG46750.024.39469515
CGTCTGA46850.024.15057216
CGTATGC47600.021.3862844
GTCACGA55450.020.24268329
TCGGAAG58750.020.0670193
ATCGGAA58450.019.7851432
CACGTCT61050.018.93854114
CACACGT64800.018.01612512
CAGTCAC66700.017.23312827
ACACGTC70300.016.86259313
TCACGAT69600.016.0949330
GTATGCC66100.015.77550645
TCTCGTA64800.015.63906341
GATCGGA75150.015.5404821
GAGCACA84650.014.4557629
TCAGATC78750.014.37257236
GTCTGAA78950.014.35995517
Produced by FastQC (version 0.11.5)