FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24686305
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42100.024.55333942
TCGTATG41250.024.4041443
ACGTCTG72500.023.14579215
CGTCTGA75500.022.25588616
GTCACAC77000.021.12121229
CGTATGC50000.018.87226344
TCGGAAG91600.018.7898923
ATCGGAA93700.018.3447632
CAGTCAC90350.018.22443827
CACGTCT95600.017.88244814
CACACGT98550.017.34715312
AGTCACA95000.017.28504628
AATCTCG77850.016.65730539
CACACTT100750.016.3878631
TTGAATC104100.015.520823536
ACACGTC111950.015.39132213
TCACACT108700.015.35485130
ACTTGAA109150.015.33838734
GAATCTC103750.014.75351938
ACACTTG114850.014.65061732
Produced by FastQC (version 0.11.5)