FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19475394
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC576340.29593239551405226TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT111400.026.60382542
TCGTATG109500.026.24278543
GTATGCC108700.025.98013145
CGTATGC108850.025.77881444
ACGTCTG144800.025.43055715
CGTCTGA146450.025.15940516
ATCGGAA156450.023.9591962
TCGGAAG159750.023.4783483
CACACGT162350.023.05562212
CACGTCT161200.022.9968314
ACACGTC166250.022.58241813
GATCGGA168200.022.3013671
TCTCGTA134300.022.28383641
CAGTCAC167050.022.00297227
AGTCACC170400.021.47799728
GTCTGAA178150.020.87231417
CGGAAGA185250.020.3922564
CCAGTCA182300.020.1993726
TCCAGTC185250.019.97484825
ACCAGAT188050.019.451132
Produced by FastQC (version 0.11.5)